Poly(A) Polymerase, Yeast
| Cat. No. | E017 | ||||||||||
| Name | Poly(A) Polymerase, Yeast | ||||||||||
| Unit | 100 U (100 μl) | ||||||||||
| Category | Molecular Biology Enzymes and Kits | ||||||||||
| Description |
Poly(A) Polymerase, Yeast catalyzes the template-independent addition of adenosine residues to the 3’ ends of RNA molecules using ATP as a substrate to create a poly(A) tail. When cordycepin-5'-triphosphate (3’-dATP) is substituted for ATP, the enzyme adds a single dA residue to the 3’-terminus of RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast is particularly effective for oligonucleotide-labeling and poly(A) tailing of long RNA templates, making it a superior choice compared to E. coli-derived enzymes. This enzyme is ideal for applications that require precise polyadenylation, such as RNA sequencing and cDNA synthesis.
|
||||||||||
| Application |
|
||||||||||
| Concentration | 1 U/μl | ||||||||||
| Components | Enzyme supplied with 5X Reaction Buffer. | ||||||||||
| Storage Buffer | 20 mM Tris-HCl (pH 8.0), 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton® X-100 and 50% (v/v) Glycerol. | ||||||||||
| Storage Condition | Store all components at -20°C. | ||||||||||
| Note |
One unit is defined as the amount of Poly(A) Polymerase, Yeast that catalyzes the incorporation of 1 nmol of AMP into RNA in 10 minutes at 37°C. |
||||||||||
| Caution | This product is distributed for laboratory research only. Not for diagnostic use . | ||||||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E017 |
| What are the key differences between Yeast and E. coli Poly(A) Polymerase? | |||||||||||||||||||
|
| Can I use dATP or ADP as a substitute for ATP in the reaction? | |
|
No, Poly(A) Polymerase from yeast specifically requires ATP for polyadenylation. While cordycepin-5’-triphosphate (3’-dATP) can be used for 3’-end labeling, neither ADP nor dATP function as substrates.
|
| How do I stop the reaction after poly(A) tailing? | |
|
The reaction can be terminated by heating at 65°C for 20 minutes or by adding 5 mM EDTA.
|
| Can I use labeled ATP for poly(A) tailing? | |
|
Yes, radiolabeled, biotinylated, or fluorescently labeled ATP can be substituted in the reaction for specific experimental applications.
|
| What are the storage conditions for this enzyme? | |
|
Store all components at -20°C and avoid repeated freeze-thaw cycles to maintain maximum performance. The components are stable for one year from the date of shipping when stored properly.
|
| What is the source of this enzyme? | |
|
The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
|
- Yoon, Y. M., Go, G., Yoon, S., Lim, J. H., Lee, G., Lee, J. H., & Lee, S. H. (2021). Melatonin Treatment Improves Renal Fibrosis via miR-4516/SIAH3/PINK1 Axis. Cells, 10(7), 1682. https://doi.org/10.3390/cells10071682
-
Alghamdi, T. A., Batchu, S. N., Hadden, M. J., Yerra, V. G., Liu, Y., Bowskill, B. B., ... & Advani, A. (2018). Histone H3 serine 10 phosphorylation facilitates endothelial activation in diabetic kidney disease. Diabetes, 67(12), 2668-2681. https://doi.org/10.2337/db18-0124
Özkan, H., Keçeli, H. H., Kaya, U., Dalkiran, S., Yüksel, M., Tek, E., & Yakan, A. (2024). Considering potential roles of selected MicroRNAs in evaluating subclinical mastitis and Milk quality in California mastitis test (+) and infected bovine milk. Animal Science Journal, 95(1), e13959. https://doi.org/10.1111/asj.13959
Sönmez, G., & İnal, Ş. (2022). microRNA expression patterns associated with average daily weight gain in Kangal. https://hdl.handle.net/20.500.12395/45671