RNase A
| Cat. No. | G117 |
| Name | RNase A |
| Unit | 25 mg |
| Category | Molecular Biology Enzymes and Kits |
| Description |
RNase A is a pancreatic bovine endoribonuclease that cleaves single-stranded RNA by hydrolysis at cytosine and uracil residues. This enzyme is highly effective for the removal of RNA from plasmid DNA preparations, making it an essential tool in nucleic acid purification protocols. RNase A helps ensure clean DNA samples by efficiently degrading contaminating RNA. |
| Application |
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| Concentration | A stock solution is recommended to be prepared in water at 10 mg/ml. Final usage concentration should be determined by end user based on specific application and usage. |
| Storage Condition |
Store all components at -20°C . |
| Note |
This product is distributed for laboratory research only Caution: Not for diagnostic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G117 |
| What is the medium of dissolving the powder (RNase)? | |
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We dissolve RNAse A in ddH2O to make stock solution of 1mg/ml. We do not have a special protocol for dissolving RNAses in other buffers.
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| After dissolving your RNase A (powder) in solution, should I boil it at 100 C for 15-20 minutes to inactivate DNase contamination? | |
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In order to remove any remaining DNase activity in the solution without compromising RNase A activity, we would suggest boiling the solution for 15 – 20 minutes. This is sufficient for most downstream molecular biology work e.g. preparation of RNA free plasmid DNA.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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Hübner, S., Sisou, D., Mandel, T., Todesco, M., Matzrafi, M., & Eizenberg, H. (2022). Wild sunflower goes viral: Citizen science and comparative genomics allow tracking the origin and establishment of invasive sunflower in the Levant. Molecular Ecology, 31(7), 2061-2072. https://doi.org/10.1111/mec.16380