Anti-CFP Tag Antibody (Monoclonal)
| Cat. No. | G164 |
| Name | Anti-CFP Tag Antibody (Monoclonal) |
| Clone | 1218 |
| Category | Tag Antibodies |
| Application | WB,ELISA,IP |
| Raised In | Mouse |
| Clonality | Monoclonal |
| Reactivity | Other |
| Description |
Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. |
| Immunogen | GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa) |
| Isotype | IgG |
| Concentration | 100ug/100ul |
| Recommended Dilutions |
WB 1:1000. For other applications, the researcher should determine the optimal working dilution. |
| Storage Buffer | PBS, pH 7.4 with 0.02% sodium azide. |
| Storage Condition |
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Specificity | Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP. |
| Purity | Affinity purified |
| Guarantee |
abm guarantees that all our Anti-CFP Tag Antibody (Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G164 |
- Lamberti, AL et al. "New insights on the interaction between the isoforms 1 and 2 of human translation elongation factor 1A" Biochimie :1-7 (2015). DOI: 10.1016/j.biochi.2015.07.021.