Anti-GFP Tag Antibody (Mouse Monoclonal)
| Cat. No. | G096 |
| Name | Anti-GFP Tag Antibody (Mouse Monoclonal) |
| Clone | LGB-1 |
| Category | Tag Antibodies |
| Application | WB,ELISA,Other |
| Raised In | Mouse |
| Clonality | Monoclonal |
| Reactivity | Other |
| Description |
Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. |
| Immunogen | GFP from the jellyfish Aequorea victoria N-Terminal peptide-KLH conjugates (first 20 aa). |
| Isotype | IgG1 |
| Concentration | 100ug/100ul |
| Recommended Dilutions |
WB 1:1000. For other applications, the researcher should determine the optimal working dilution. |
| Storage Buffer | PBS, pH 7.4 with 0.05% sodium azide. |
| Storage Condition |
This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
| Formulation | Liquid |
| Specificity | Recognizes native and denatured forms of GFP and its variants EGFP, YFP, EYFP, and CFP. |
| Purity | Affinity purified |
| Guarantee |
abm guarantees that all our Anti-GFP Tag Antibody (Mouse Monoclonal) will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G096 |
| Have the antibody been tested for Immunoprecipitation? | |
|
Yes, and it works fine.
|
- Moncivais, K et al. "Tetracycline repressor-based mammalian two-hybrid systems" Methods Mol Biol 812:259-273 (2012). DOI: 10.1007/978-1-61779-455-1_15. PubMed: 22218865. Application: Western Blot.
- Kim, K et al. "Suppression of OsKu80 results in defects in developmental growth and increased telomere length in rice (Oryza sativa L.)" Biochem Biophys Res Commun 4:857-862 (2015). PubMed: 26590017.
- Feng, Y., Hu, Y., Hou, Z., Sun, Q., Jia, Y., & Zhao, R. "Chronic corticosterone exposure induces liver inflammation and fibrosis in association with m 6 A-linked post-transcriptional suppression of heat shock proteins in chicken" Cell Stress and Chaperones 1-10: (2019).
- Segal, I., Nachmias, D., Arbely, E., & Elia, N. "A straightforward approach for bioorthogonal labeling of proteins and organelles in live mammalian cells, using a short peptide tag" : (2019). DOI: 10.1101/708545.