Anti-NF-kB p65 (Phospho-Thr254) Antibody
Cat. No. | Y011010 |
Name | Anti-NF-kB p65 (Phospho-Thr254) Antibody |
Clone | N/A |
Category | Polyclonal Antibodies |
Gene Name | NF-kB p65 |
Application | WB,IHC |
Application Info | WB: 1:500~1:1000; IHC: 1:50~1:100 |
Raised In | Rabbit |
Clonality | Polyclonal |
Immunogen | The antiserum was produced against synthesized phosphopeptide derived from human NF-κB p65 around the phosphorylation site of threonine 254 (F-R-TP-P-P). |
Concentration | 1ug/ul |
Recommended Dilutions | WB: 1:500~1:1000; IHC: 1:50~1:100 |
Storage Buffer | phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Storage Condition | This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt. |
Formulation | Liquid |
Species Reactivity | Human,Mouse,Rat |
Specificity | NF-κB p65 (phospho-Thr254) antibody detects endogenous levels of NF-κB p65 only when phosphorylated at threonine 254. |
Purity | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. |
Guarantee |
abm guarantees that all our Anti-NF-kB p65 (Phospho-Thr254) Antibody will perform as described on this product webpage, if this is not the case we will provide you with a one-time replacement at no extra cost. Documentation and explanation of experiment conducted will be required when submitting a claim for replacement. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Y011010 |
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- Jadhav, A et al. "Treatment with heme arginate alleviates adipose tissue inflammation and improves insulin sensitivity and glucose metabolism in a rat model of Human primary aldosteronism" Free Radic Biol Med 53:2277-86 (2012). PubMed: 23089228.
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