Ni-IDA Agarose Resins
Cat. No. | G253 |
Name | Ni-IDA Agarose Resins |
Category | Buffers & General Chemicals |
Description |
Affinity chromatography is a method of separating biochemical mixtures based on highly specific molecular interactions. The Ni-IDA agarose is developed for the purification of proteins with an affinity tag of six consecutive histidine residues. This histidine-metal ion interaction makes one-step purification possible for proteins from any expression system, under native or denaturing conditions. The His-tag sequence binds to Ni2+ cations, which are immobilized onto a solid support using iminodiacetic acid groups (IDA). Impurities are removed and the purified His-tagged proteins can be eluted by imidazole or a reduced pH solution.
Special Features
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Application |
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Storage Condition |
Resin should be stored at 2-8°C. Do not freeze. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G253 |
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Does Cat# G253 Ni-IDA Agarose Resins for Protein Purification contain ethyl alcohol? | |
Yes, this product contains ethyl alcohol.
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