Recombinant Human Artemin (ARTN) (E. coli)

Cat. No.

Z102355

Unit

Price

$290.00

Cat. No.	Z102355
Name	Recombinant Human Artemin (ARTN) (E. coli)
Description	Artemin is a disulfide-linked member of the glial cell line-derived homodimeric neurotrophic factor (GDNF) family which all signal via a multicomponent receptor system composing of a high-affinity binding component (GFRα1-4) and a common signalling receptor tyrosine kinase (RET). Artemin prefers the receptor GFRα3-RET, but will use the others as an alternative. Artemin promotes the survival and growth of peripheral and central neurons, including dopaminergic neurons in the mid-brain; and plays an important role in the development of sensory and sympathetic neurons.
Organism	Human (H. sapiens)
Unit quantity	20 µg
Family	TGF-beta Super-family
Tag	Tag Free
Aliases	ART, ENOVIN, EVN, NBN
Gene Symbol	ARTN
Gene ID	9048
Accession Number	Q5T4W7
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	12.2 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered 10mM sodium citrate pH-4.5 and 25mM sodium chloride solution
Function	The ED50 as determined by its binding ability in a functional ELISA (using SH-SY5Y human neuroblastoma cells), is 4-16 ng/mL
Reconstitution	A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102355

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.