Recombinant Human Fractalkine (CX3CL1) (E. coli)

Cat. No.

Z101895

Unit

Price

$212.00

Cat. No.	Z101895
Name	Recombinant Human Fractalkine (CX3CL1) (E. coli)
Description	Fractalkine is a type I membrane protein that signals through the CX3CR1 receptor. The extracellular domain of Fractalkine is released by protease cleavage – producing soluble Fractalkine. Fractalkine has been found in many tissues and shown to be up-regulated in endothelial cells and microglia in response to inflammatory signals. Fractalkine is also involved in leukocyte adhesion as well as attracting monocytes, microglia cells and NK cells.
Organism	Human (H. sapiens)
Unit quantity	20 µg
Family	Chemokine-alpha Family (CXC)
Tag	Tag Free
Aliases	ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin
Gene Symbol	CX3CL1
Gene ID	6376
Accession Number	P78423
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	8.9 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2µm filtered solution in 20mM phosphate buffer and 50mM NaCl, pH 7.4
Function	Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED(50) for this effect is typically 20100 ng/mL
Reconstitution	A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101895

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	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.