Recombinant Human HB-EGF (Heparin Binding EGF) (E. coli)

Cat. No.

Z102155

Unit

Price

$92.00

Cat. No.	Z102155
Name	Recombinant Human HB-EGF (Heparin Binding EGF) (E. coli)
Description	Human HBEGF (Heparin Binding EGF like growth factor) is a member of the EGF family of peptide growth factors that signal through the EGF receptor, and stimulates the proliferation of smooth muscle cells, fibroblasts, epithelial cells, and keratinocytes. HBEGF is expressed in many cell types and tissues, including bronchial epithelium, visceral and vascular smooth muscle, CD4+ T cells, glomerular podocytes, keratinocytes, macrophages and certain tumor cells. Mature HBEGF is a soluble peptide that arises from the proteolytic processing of the 208 amino acid type-I transmembrane form and exhibits the ability to specifically bind heparin and heparin sulfate proteoglycans.
Organism	Human (H. sapiens)
Unit quantity	50 µg
Family	EGF Family
Aliases	DTR, DTS, DTSF, HEGFL
Gene Symbol	HBEGF
Gene ID	1839
Accession Number	Q99075
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	10.1 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Function	The ED50, as determined by a cell proliferation assay (using balb/c 3T3 cells) is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Reconstitution	It is recommended to reconstitute HBEGF in sterile PBS at 250 μg/mL.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102155

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.