Recombinant Human IL21 (E. coli)

Cat. No.

Z100495

Unit

Price

$293.00

Cat. No.	Z100495
Name	Recombinant Human IL21 (E. coli)
Description	IL-21 stimulates proliferation of B cell stimulated by crosslinking of the CD40 antigen. It inhibits proliferation stimulated by IL-4 plus anti-IgM. IL-21 augments stimulation of the proliferation of naive CD45RA(+) but not memory CD45R0(+) T cells mediated by engagement of CD3. IL-21 stimulates the proliferation of bone marrow progenitor cells and the expression of the natural killer cell marker CD56 in the presence of IL-15. The Interleukin-21 receptor is closely related to human IL-2 beta receptor. The receptor contains a WSXWS motif in the extracellular region, typical of type 1 cytokine receptors. The receptor is expressed on natural killer cells, T cells, and B cell line. The functional signaling complex activates Janus kinases JAK1, JAK3, and the STAT proteins STAT1, and STAT3.
Organism	Human (H. sapiens)
Unit quantity	10 µg
Family	Other Interleukins
Aliases	CVID11, IL-21, Za11
Gene Symbol	IL21
Gene ID	59067
Accession Number	Q9HBE4
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	15 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant Interleukin-21 was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.
Function	The ED(50) was determined by the dose-dependent proliferation of HuT-78 Cells and was found to be <0.05ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100495

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.