Recombinant Human Persephin (PSPN) (E. coli)

Cat. No.

Z102495

Unit

Price

$290.00

Cat. No.	Z102495
Name	Recombinant Human Persephin (PSPN) (E. coli)
Description	Persephin is a disulfide-linked homodimeric neurotrophic factor belonging to the Transforming Growth Factor-beta (TGF-beta) superfamily and the GDNF sub-family. One cysteine residue, out of the seven conserved, is used for inter-chain disulfide bridging while the others form a cysteine knot ring formation. It signals through RET and one of the four GFR-alpha (alpha-1, alpha-2, alpha-3, alpha-4) receptors to promote the proper development and maintenance of the nervous system. Persephin promotes survival of dopaminergic neurons in the midbrain as well as some motor neurons, and promotes branching of ureteric buds.
Organism	Human (H. sapiens)
Unit quantity	20 µg
Family	TGF-beta Super-family
Aliases	PSP
Gene Symbol	PSPN
Gene ID	5623
Accession Number	O60542
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	10.7 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution in Acetonitrile and TFA (with BSA as a carrier protein)
Function	The ED50 is 4.0-16.0 ng/mL as determined by a cell proliferation assay
Reconstitution	Reconstitute in sterile 4mM HCl at 100µg/mL (containing at least 0.1% human or bovine serum albumin)
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102495

Print/Download Datasheet

Search CoA here

MSDS

Growth Factors, Cytokines & Kinases MSDS

Other

How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.