Recombinant Human PlGF (E. coli)

Cat. No.

Z102615

Unit

Price

$113.00

Cat. No.	Z102615
Name	Recombinant Human PlGF (E. coli)
Description	Human PlGF, or Placental Growth Factor, is part of the PDGF/VEGF family and contains the 8 cysteine residues conserved in the family. It can be alternately spliced into three different mature forms. PGF is typically found as a disulfide linked dimer which is highly glycosylated. It is a key molecule involved in vasculogenesis and angiogenesis, especially during embryogenesis. It also induces the activation of monocytes, as well as their migration and production of inflammatory cytokines. It is primarily expressed by the placental trophoblast during pregnancy, but is also expressed in villious trophoblasts, decidual cells, erythroblasts, keratinocytes, and somewhat in endothelial cells. PGF signals by binding VEGF R1, and competes with VEGF for binding. Recombinant PlGF is a 15.0 kDa protein.
Organism	Human (H. sapiens)
Unit quantity	25 µg
Family	VEGF/PGDF/Pleiotrophin Family
Aliases	D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760
Gene Symbol	PGF
Gene ID	5228
Accession Number	P49763
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	15 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution in Citrate, phosphate and NaCl (pH 4.0)
Function	Not available yet
Reconstitution	A quick spin of the vial followed by reconstitution in PBS to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102615

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MSDS

Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.