Recombinant Human RANKL (TNFSF11) (E. coli)

Cat. No.

Z102265

Unit

Price

$320.00

Cat. No.	Z102265
Name	Recombinant Human RANKL (TNFSF11) (E. coli)
Description	Human RANKL is a member of the tumour necrosis factor superfamily. It primarily affects the immune system by controlling bone regeneration and remodeling. It functions in osteoclast differentiation and activation. It also is expressed by T helper cells as a factor in dendritic cell maturation and survival. It is expressed after T-cell activation. It also appears to have a role in the regulation of apoptosis through activation of the antiapoptotic kinase AKT/PKB. It is not only expressed by immune cells, but also on a number of other cells in the lung, thymus, skeletal muscle and many others. It can be expressed as a trimeric transmembrane protein, a secreted form or a truncated ectodomain. Human RANKL shares 85% sequence similarity with mouse RANKL.
Organism	Human (H. sapiens)
Unit quantity	10 µg
Family	TNF Super-family
Aliases	CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf
Gene Symbol	TNFSF11
Gene ID	8600
Accession Number	O14788
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	20 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Function	coming soon
Reconstitution	A quick spin of the vial followed by reconstitution in sterile distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102265

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.