Recombinant Human Resistin (RETN) (E. coli)
Cat. No. | Z100805 |
Name | Recombinant Human Resistin (RETN) (E. coli) |
Description | Resistin was identified by screening for genes that are induced during the differentiation of adipocytes but down-regulated in mature adipocytes. Resistin gene expression is induced during the differentiation of adipocytes. Resistin circulates in mouse serum, and its level is increased markedly in obesity. Adipocytes secrete resistin as a unique signaling molecule that may be the hormone potentially linking obesity to type II diabetes, which is characterized by target-tissue resistance to insulin. TNF-alpha is a negative regulator of Resistin gene expression and inhibits Resistin mRNA expression and protein secretion by 70-90% in 3T3-L1 adipocytes. |
Organism | Human (H. sapiens) |
Unit quantity | 25 µg |
Family | FIZZ(Resistin)/Leptin Families |
Aliases | ADSF, FIZZ3, RENT, RETN1, RSTN, XCP1 |
Gene Symbol | RETN |
Gene ID | 56729 |
Accession Number | Q9HD89 |
Source | E. coli |
Species | Human (H. sapiens) |
Appearance | Lyophilized Powder |
Molecular Weight | 10 kDa |
Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method. |
Purity | >95% as determined by SDS-PAGE |
Formulation | Recombinant human Resistin was lyophilized from a 0.2 μm filtered PBS solution. |
Function | Determined by its ability to stimulate lipolysis in cultured human adipocytes. |
Reconstitution | A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. |
Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
Usage | For research use only. Not for diagnostic or therapeutic use. |
Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z100805 |
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What is the shelf life and storage condition of this product? | |
The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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