Recombinant Human SHH (E. coli)

Cat. No.

Z101675

Unit

Price

$113.00

Cat. No.	Z101675
Name	Recombinant Human SHH (E. coli)
Description	Highly conserved proteins, members of the Hedgehog family are widely represented throughout the animal kingdom. The three known mammalian Hedgehog proteins; Sonic (SHH), Desert (DHH) and Indian (IHH) hedgehog share a high degree of sequence and structural identity. SHH is specifically known to be expressed in fetal intestine, liver, lung and kidney. Recombinant Human SHH represents the N-product (N-terminal domain with Cys24 substituted by an Ile-Val-Ile sequence) which retains all the known signalling capabilities.
Organism	Human (H. sapiens)
Unit quantity	30 µg
Family	Hedgehog Family
Aliases	HHG1, HLP3, HPE3, MCOPCB5, SMMCI, ShhNC, TPT, TPTPS
Gene Symbol	SHH
Gene ID	6469
Accession Number	Q15465
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	20.1 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 μm filtered solution in Phosphate buffer and NaCl
Function	Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 0.1 - 0.4 µg/mL.
Reconstitution	A quick spin of the vial followed by reconstitution at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z101675

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.