Recombinant Human TGF Beta-3 (TGFB3) (E. coli)
| Cat. No. | Z102515 |
| Name | Recombinant Human TGF Beta-3 (TGFB3) (E. coli) |
| Description |
Transforming Growth Factor Beta-3 is a disulfide-linked homodimeric, non-glycosylated, protein that is one of the isoforms of the highly pleiotropic cytokines that are secreted by all cell types. TGF Beta-1, TGF Beta-2, and TGF Beta-3 use the same receptor and act as cellular switches that regulate cell growth, proliferation, differentiation, motility, extracellular matrix synthesis, and immune function regulation. Defects of this gene can cause arrhythmogenic right ventricular dysplasia 1 and rienhoff syndrome. TGFB3 first interacts with the accessory receptor betaglycan (TGF Beta-R3) and a type II serine/threonine kinase receptor (TGF Beta-R2). The receptor then activates a type I serine/threonine kinase receptor such as ALK-1 or TGF Beta-R1 (ALK-5) through phosphorylation, and the activated type I receptor activates Smad proteins to regulate transcription. |
| Organism | Human (H. sapiens) |
| Unit quantity | 10 µg |
| Family | TGF-beta Super-family |
| Aliases | ARVD, ARVD1, LDS5, RNHF, TGF-beta3 |
| Gene Symbol | TGFB3 |
| Gene ID | 7043 |
| Accession Number | P10600 |
| Source | E. coli |
| Species | Human (H. sapiens) |
| Appearance | Liquid |
| Molecular Weight | 13 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
| Purity |
>95% as determined by SDS-PAGE |
| Formulation | The filter sterilized solution contains 20% Ethanol and 0.06% Acetic acid. |
| Function | The ED50 is 0.01-0.04 ng/mL as determined by its ability to inhibit IL-4 dependent proliferation of HT-2 mouse cells |
| Storage | This cytokine can be stored in working aliquots at 2° - 8°C for two weeks. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102515 |
| How do I choose the right growth factor for my experiment? | |
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| What is the shelf life and storage condition of this product? | |
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The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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