Recombinant Human VEGFB (E. coli)

Cat. No.

Z102535

Unit

25 µg

Price

$891.00

Cat. No.	Z102535
Name	Recombinant Human VEGFB (E. coli)
Description	Vascular endothelial growth factor B (VEGFB) is a homodimeric, heparin-binding glycoprotein member of the VEGF family of growth factors. Compared to VEGFA, VEGFB’s main role is the maintenance of newly formed blood vessels and is involved in endothelial cell physiology. It is a ligand for vascular endothelial growth factor receptor 1 (VEGFR1) and neuropilin-1 (NRP1). VEGFB (amino acids 22-207) is fused to a His tag that adds 21 amino acids to the N-terminus.
Organism	Human (H. sapiens)
Unit quantity	25 µg
Family	VEGF/PGDF/Pleiotrophin Family
Aliases	VEGFL, VRF
Gene Symbol	VEGFB
Gene ID	7423
Accession Number	P49765
Source	E. coli
Species	Human (H. sapiens)
Appearance	Lyophilized Powder
Molecular Weight	19 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	0.2 µm filtered solution in 20 mM Tris-HCl buffer pH 7.5 and 10% glycerol
Function	Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is < 1ng/ml.
Storage	Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage, we recommend adding a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z102535

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MSDS

Growth Factors, Cytokines & Kinases MSDS

Other

How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.