Recombinant Mouse G-CSF (CSF3) (E. coli)
| Cat. No. | Z200605 |
| Name | Recombinant Mouse G-CSF (CSF3) (E. coli) |
| Description |
Granulocyte colony-stimulating factor (G-CSF) is a growth factor and cytokine belonging to the IL-6 family of interleukins. It is produced by a variety of cells such as: endothelial cells, bone marrow stroma, macrophages, fibroblasts, and other immune cells in response to endotoxin activation. G-CSF stimulates the bone marrow to stimulate the survival, proliferation and differentiation of progenitor cells to mature neutrophils while enhancing its activities. It indirectly mobilizes stem cells by downregulating the expression of CXCL12 on marrow osteoblasts and releases neutrophil and monocyte proteolytic enzymes. Mouse G-CSF affects mouse skeletal muscle development by activating proliferation of myoblasts. Human and murine G-CSF shares a 73% amino acid identity and is cross-reactive. |
| Organism | Mouse (M. musculus) |
| Unit quantity | 10 µg |
| Family | IL-6 Family |
| Aliases | Csfg, G-CSF, MGI-IG |
| Gene Symbol | CSF3 |
| Gene ID | 12985 |
| Accession Number | P09920 |
| Source | E. coli |
| Species | Mouse (M. musculus) |
| Appearance | Lyophilized Powder |
| Molecular Weight | 19.2 kDa |
| Endotoxin Level | <1.0 EU/µg of recombinant protein as determined by the LAL method |
| Purity | >95% as determined by SDS-PAGE |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Function | The ED50 was 10.0-60.0 pg/mL as determined by a cell proliferation assay |
| Reconstitution | Reconstitute in sterile deionized water at 100 µg/mL |
| Storage | The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles. |
| Usage | For research use only. Not for diagnostic or therapeutic use. |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200605 |
| How do I choose the right growth factor for my experiment? | |
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| What is the shelf life and storage condition of this product? | |
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The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
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