Recombinant Mouse LIX (CXCL5) (E. coli)

Cat. No.

Z200555

Unit

Price

$92.00

Cat. No.	Z200555
Name	Recombinant Mouse LIX (CXCL5) (E. coli)
Description	Epithelial neutrophil- activating protein 78 (ENA-78) is a member of the CXC chemokines and acts as a potent chemoattractant and activator of neutrophil function. On stimulation in vitro, ENA-78 is highly expressed in many cell types. ENA-78 protein levels are strongly elevated in synovial fluid and blood of patients with rheumatoid arthritis. By in situ hybridization and immunofluorescence staining, ENA-78 has been recognized as a major CXC chemokine expressed in epithelial cells of the intestinal mucosa of patients with Crohn's disease, ulcerative colitis, and acute appendicitis.
Organism	Mouse (M. musculus)
Unit quantity	20 µg
Family	Chemokine-alpha Family (CXC)
Aliases	AMCF-II, Cxcl6, ENA-78, GCP-2, LIX, Scyb5, Scyb6
Gene Symbol	CXCL5
Gene ID	20311
Accession Number	P50228
Source	E. coli
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	11 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 µm filtered solution in 20 mM PB,100 mM NaCl pH 7.5.
Function	Determined by its ability to chemoattract human neutrophils using a concentration range of 2.0-40 ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200555

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Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

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	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.