Recombinant Mouse MCP-5 (CCL12) (E. coli)

Cat. No.

Z200385

Unit

Price

$92.00

Cat. No.	Z200385
Name	Recombinant Mouse MCP-5 (CCL12) (E. coli)
Description	MCP-5 (CCL12) is a chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. It is also a potent active monocyte chemokine that signals through CCR2. It is Involved in allergic inflammation and the host response to pathogens. Also, it may play a pivotal role during early stages of allergic lung inflammation.
Organism	Mouse (M. musculus)
Unit quantity	25 µg
Family	Chemokine-beta Family (CC)
Tag	Tag Free
Aliases	MCP-5, Scya12
Gene Symbol	CCL12
Gene ID	20293
Accession Number	Q62401
Source	E. coli
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	10 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant MCP-5 was lyophilized from a 0.2 μm filtered 20 mM PB solution pH 7.5, 100 mM NaCl.
Function	Determined by its ability to chemoattract human monocytes using a concentration range of 20-40 ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200385

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Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

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	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.