Recombinant Mouse Noggin (NOG)

Cat. No.

Z200235

Unit

20 µg

Price

$320.00

Cat. No.	Z200235
Name	Recombinant Mouse Noggin (NOG)
Description	Noggin is a secreted homodimeric glycoprotein that binds to ligands of the TGF-beta family (BMPs) and regulate their activity by inhibiting their access to signaling receptors. Mature human Noggin protein contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine knot structure. Noggin binds different BMPs with variable affinities, antagonizing specific BMPs during skeletal development. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. Targeted deletion of Noggin in mice is known to result in severely malformed skeletal system and prenatal death while over-expression of Noggin in mature osteoblasts has been associated with severe osteoporosis.
Organism	Mouse (M. musculus)
Unit quantity	20 µg
Family	Other Growth Factors/Cytokines
Aliases	DCYTB, FLJ23462, FRRS3
Gene Symbol	NOG
Gene ID	18121
Accession Number	P97466
Source	E. coli
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	23 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method
Purity	>95% as determined by SDS-PAGE
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS (pH 7)
Function	The ED50 as determined by induced alkaline phosphatase secretion in ATDC cells was determined to be ≤ 2.0 ng/mL
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200235

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.