Recombinant Mouse Prolactin (PRL)

Cat. No.

Z200495

Unit

10 µg

Price

$320.00

Cat. No.	Z200495
Name	Recombinant Mouse Prolactin (PRL)
Description	Prolactin is a neuroendocrine pituitary hormone of 23 kDa. It is produced in increasing amounts during pregnancy and during suckling and acts primarily on the mammary gland by initiating and maintaining lactation in the postpartal phase. Prolactin has been shown also to have cytokine-like activities and to have important immunoregulatory activities. It contributes to the development of lymphoid tissues and the maintenance of physiological immune function and also modulates a variety of T cell immune responses. In addition to triggering resting lymphocytes to cell division, the hormone can also control the magnitude of their response to polyclonal stimuli. Prolactin promotes the proliferation of Nb2 pre-T cell lymphoma cells. In these cells prolactin induces the biphasic expression of a transcription factor, IRF1 and may be involved in cell cycle activation and S phase progression. Prolactin has been reported to activate cellular proliferation in non-reproductive tissue, such as liver, spleen, and thymus. It induces significant proliferation in aortic smooth muscle cells and also enhances proliferation of these cells induced by PDGF.
Organism	Mouse (M. musculus)
Unit quantity	10 µg
Family	Somatotropin/Sclerostin/Serpin Families
Aliases	Gha1, Prl1a1
Gene Symbol	PRL
Gene ID	19109
Accession Number	P06879
Source	Insect cells
Species	Mouse (M. musculus)
Appearance	Lyophilized Powder
Molecular Weight	23 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant mouse Prolactin is presented as concentrated 1mg/ml, 0.2 μm filtered 20 mM Tris solution, 0.5 M NaCl pH 7.4, containing 5% trehalose 10% glycerol.
Function	The activity was determined by the dose-dependent proliferation of Nb2 cells.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration of 0.1 mg/mL.
Storage	Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z200495

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.