Recombinant Rat IL6

Cat. No.

Z300085

Unit

Price

$320.00

Cat. No.	Z300085
Name	Recombinant Rat IL6
Description	IL-6 is produced by many different cell types. The main sources in vivo are stimulated monocytes, fibroblasts, and endothelial cells. Macrophages, T cells and B lymphocytes, granulocytes, smooth muscle cells, eosinophils, chondrocytes, osteoblasts, mast cells, glial cells, and keratinocytes also produce IL-6 after stimulation. IL-6 is a member of a family of cytokines, which also includes LIF, CNTF, Oncostatin M, IL-11, and CT-1. All known members of the IL-6 cytokine family induce hepatic expression of acute phase proteins. The IL-6 receptor is expressed on T cells, mitogen-activated B cells, peripheral monocytes and some macrophage and B cell derived tumor cell types. It is not expressed in resting B cells but in resting T cells. The IL-6 receptor is a strongly glycosylated protein of 80 kDa and a length of 449 amino acids. It has been designated CD126.
Organism	Rat (R. norvegicus)
Unit quantity	10 µg
Family	IL-6 Family
Tag	Tag Free
Aliases	ILg6, Ifnb2
Gene Symbol	IL6
Gene ID	24498
Accession Number	P20607
Source	E. coli
Species	Rat (R. norvegicus)
Appearance	Lyophilized Powder
Molecular Weight	22 kDa
Endotoxin Level	<1.0 EU/µg of recombinant protein as determined by the LAL method.
Purity	>95% as determined by SDS-PAGE
Formulation	Recombinant rat Interleukin-6 was lyophilized from a 0.2 μm filtered PBS solution.
Function	The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20-40 ng/mL.
Reconstitution	A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage	For research use only. Not for diagnostic or therapeutic use.
Material Citation	If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. Z300085

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Growth Factors, Cytokines & Kinases MSDS

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How do I choose the right growth factor for my experiment?

Cell type: Different cells respond to different growth factors. Choose a growth factor that promotes the growth or differentiation of your target cell type.
Desired outcome: Whether you are studying proliferation, differentiation, or survival, different growth factors will promote different cellular behaviors.
Concentration and formulation: Different experiments may require varying concentrations of growth factors.

Check out Role of Growth Factors in Cell Differentiation.

	What is the shelf life and storage condition of this product?
	The lyophilized protein is stable for at least one year from date of receipt at -70°C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.