saCas9 Nuclease NLS Protein
| Cat. No. | K189 | |
| Name | saCas9 Nuclease NLS Protein | |
| Unit | 32.5 µg (250 pmol/50 µL) | |
| Category | Cas Proteins & CRISPR Screening | |
| Description |
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.
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| Cas Type | Nuclease | |
| Cas Origin | saCas9 | |
| Cas Protein Marker | No GFP | |
| Concentration | 5 µM, 0.65 mg/ml | |
| Format General | Enzyme supplied with 10X Reaction Buffer | |
| Endotoxin Level | <1.0 EU/μg of recombinant protein as determined by the LAL method. | |
| Storage Buffer | 20mM HEPES, pH 7.5, 100mM KCl, 1mM DTT, 0.1mM EDTA and 50% (v/v) Glycerol. | |
| Storage Condition | Store all components at -20°C. | |
| Caution | This product is distributed for laboratory research only. Not for diagnostic use. | |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K189 |
| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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