saCas9 Nuclease Protein

Cat. No.

K044

Unit

6.5 µg (50 pmol/50 µL)

Price

$57.50

Cat. No.

K044

Name

saCas9 Nuclease Protein

Unit

6.5 µg (50 pmol/50 µL)

Category

Cas Proteins & CRISPR Screening

Description

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the latest RNA-guided endonuclease tool in genome editing which allows for very specific genomic disruption and replacement.

The Cas9 nuclease serves to unwind the genomic DNA duplex next to conserved protospacer adjacent motifs (PAMs) and homes in on its target sequence, which is recognized by a complementary single-guide RNA. The resulting double-stranded break gets repaired by the non-homologous end joining (NHEJ) pathway, leading to a disruption in the open reading frame of the targeted gene. Alternatively, by supplying a suitable repair template, virtually any desired point mutation can be introduced at the break point via homology-directed repair (HDR).

The Cas9 nuclease from the bacteria Staphylococcus aureus, abbreviated saCas9, is gaining popularity as an alternative to spCas9 due to its relatively smaller size. The saCas9 PAM sequence is 5’-NNGRRN (preferably 5’-NNGRRT).

abm offers a variety of Cas9 proteins. View our whole collection here.

Cas Type

Nuclease

Cas Origin

saCas9

Cas Protein Marker

No GFP

Concentration

1000 nM, 130 µg/ml

Format General

Enzyme supplied with 10X Reaction Buffer

Endotoxin Level

<1.0 EU/μg of recombinant protein as determined by the LAL method.

Storage Buffer

10 mM Tris-HCl (pH 7.4), 0.1 mM EDTA, 1 mM DTT, 300 mM NaCl, and 50% (v/v) Glycerol.

Storage Condition

Store all components at -20°C.

Caution

This product is distributed for laboratory research only. Not for diagnostic use.

Material Citation

If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. K044

Datasheet

saCas9 Nuclease Protein Datasheet

Search CoA here

MSDS

Cas9 Proteins MSDS

	Does K044 contain a HIS-tag?
	Yes, it does contain a His-tag.

	What is the source of this enzyme?
	The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.