Safe-Red™ Gel
| Cat. No. | G680 |
| Name | Safe-Red™ Gel |
| Unit | 1.0 ml |
| Category | Gel Documentation |
| Description |
abm’s Safe-Red™ Gel is a safe, improved formulation of nucleic acid stain for agarose gel electrophoresis. Safe-Red™ Gel directly replaces toxic Ethidium Bromide and eliminates its associated contamination risks with glassware, gel apparatus and environment. Features:
|
| Safeview Series | Gel Casting Dye |
| Stain Color | Red |
| Application |
Safe Detection of dsDNA, ssDNA and RNA in agarose gels. |
| Concentration | 10000X |
| Format General | Pre-cast or Post-stain |
| Shipping Conditions |
Shipped on blue ice packs. |
| Storage Condition |
18-25°C |
| Note |
Dispose of SafeView DNA Stains as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide). |
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G680 |
| Can SafeView products be used in replacement of ethidium bromide? | |
|
All SafeView™ products (Safe-Green™, Safe-Red™, SafeView™ Classic, Safe-Red™ Gel) can be used as a complete replacement for your ethidium bromide workflow. |
| How do SafeView products work and why are they not carcinogenic? | |
|
SafeView™ products contain fluorescent compounds that have a strong affinity to nucleic acids. Once bound to a nucleic acid, the compound fluoresces under specific wavelength of light which can then be visualized using a standard UV/Blue light imager. There may be some unknown effects of SafeView™ products that have not been documented in literature but these would also apply to equivalent popular products such as SYBRSafe. However, SafeView™ products are not as carcinogenic as ethidium bromide. |
| How do I use SafeView products? | |
|
Safe-Red™ and Safe-Green™ are supplied in a 6X loading dye format: mix samples and DNA marker with Safe-Red™ or Safe-Green™ at a 1:5 (dye : sample) dilution ratio, and load onto an unstained gel. |
| Can SafeView products differentiate between double stranded and single stranded nucleic acids? | |
|
No, our SafeView products will bind to both double-stranded and single-stranded nucleic acids, albeit with lesser efficiency for single-stranded nucleic acid species.
|
| At what temperature do I store SafeView products? | |
|
SafeView Classic™, Safe-Red™ and Safe-Red™ Gel should be stored at 18-25°C. Safe-Green™ should be stored at 4°C. |
| How sensitive are SafeView products? | |
|
Safe-Green™: 0.2-0.6 ng DNA per band |
| Can SafeView products be used to post-stain gels? | |
|
Yes, SafeView™ Classic and Safe-Red™ Gel can both be used for either pre-cast or post-stain gels.
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| Do I need to add any loading dye to my samples prior to running them on a SafeView agarose gel? | |
|
If using Safe-Green™ or Safe-Red™, you will not need to add any additional loading dyes to your samples. If using SafeView™ Classic or Safe-Red™ Gel, you will need to add an appropriate DNA loading dye to your samples in order for samples to properly settle and stay in the wells during electrophoresis. |
| I cannot visualize 100bp and 200bp DNA bands on a 1% SafeView agarose gel. What should I do? | |
|
It is often difficult to detect smaller 100bp and 200bp bands on a 1% gel especially if samples are of low concentration. We recommend visualizing smaller fragments on a higher concentration gel - ideally 2% agarose.
|
| Can I use SafeView products to visualize nucleic acids on a polyacrylamide gel? | |
|
SafeView™ products are designed for use with agarose gels, and are not optimized for polyacrylamide gels. However, SafeView™ Classic and Safe-Red™ Gel can both visualize nucleic acids on polyacrylamide gels (Acrylamide, TBE, APS, TEMED) by post staining method.
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| Can SafeView products be stored at -20°C? | |
|
No, please store Safe-Green™ at 4°C and SafeView™ Classic, Safe-Red™ and Safe-Red™ Gel at 18-25°C. |
| What is the concentration of SafeView products? | |
|
SafeView™ Classic and Safe-Red™ Gel are supplied at a 10,000X concentration. Safe-Green™ and Safe-Red™ are supplied at a 6X concentration.
|
| Can I perform gel extraction and subsequent cloning using SafeView products? | |
|
We recommend using SafeView™ Classic or Safe-Red™ Gel which can both be used for gel extraction of DNA fragments destined for cloning. We have observed no decrease in cloning efficiency in comparison to ethidium bromide.
|
| What type of device or imaging system should be used to visualize SafeView agarose gels? | |
|
We recommend using abm’s SafeViewER™ Imager (Cat.No E1001) which is compatible with all SafeView™ products. Additionally, any imaging system with UV, Blue Light and/or LED should be sufficient.
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- Gosar, A. A., Kimani, J. W., Nzou, S. M., Mwangi, A. W., Ndung’u, F., Sora, G. H., & Kimotho, J. H. (2024). Generation and Characterization of a variant of Thermostable Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) Enzyme. F1000Research, 13, 1041. https://doi.org/10.12688/f1000research.152224.1