Thermolabile UDG
Cat. No.
E095
Unit
200 reactions
Price
$88.00
| Cat. No. | E095 | ||||||
| Name | Thermolabile UDG | ||||||
| Unit | 200 reactions | ||||||
| Category | Molecular Biology Enzymes and Kits | ||||||
| Description |
Thermolabile UDG is a uracil-DNA glycosylase which catalyzes the release of uracil from uracil-containing single-stranded or double-stranded DNA. The release of uracil results in high susceptibility to hydrolytic cleavage at elevated temperatures or high pH. This function allows for many applications such as the prevention of carryover contamination during PCR or qPCR. By incorporating uracil in the reactions, the degradation of previous reaction products can be ensured and any contamination can be prevented. Furthermore, this enzyme is sensitive to heat and can be rapidly and completely inactivated at temperatures above 50°C which also prevents any downstream interference.
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E095 |
Datasheet
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| What is Thermolabile UDG used for? | |
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Thermolabile UDG (Uracil-DNA Glycosylase) is an enzyme that helps prevent carryover contamination during PCR or qPCR by catalyzing the release of uracil from uracil-containing DNA. This degradation of previous reaction products ensures that any contamination from prior PCR/qPCR reactions is removed before starting a new experiment.
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| How does Thermolabile UDG prevent contamination in PCR/qPCR? | |
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By incorporating uracil into the DNA during PCR/qPCR, Thermolabile UDG can selectively degrade uracil-containing DNA. This enzyme is inactivated by heat (above 50°C), which ensures that there is no interference from previous reactions or contamination in downstream applications.
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| Can I use Thermolabile UDG in other applications apart from PCR/qPCR? | |
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Yes, Thermolabile UDG can be used in other applications where degradation of uracil-containing DNA is required. For non-PCR/qPCR applications, it is recommended to follow the protocol using the UDG Reaction Buffer.
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| Can I directly use Thermolabile UDG in my PCR/qPCR reactions? | |
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Yes, Thermolabile UDG can be directly applied to PCR/qPCR reactions without the need for the UDG Reaction Buffer. This method is particularly useful for preventing carryover contamination from previous PCR/qPCR reactions. To do this, simply mix Thermolabile UDG with your PCR/qPCR reaction mix, and add an initial activation step at 25°C for 30 seconds. This ensures that any uracil-containing products from previous reactions are degraded before proceeding with the current experiment.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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