DNase I
| Cat. No. | G028 | ||||||
| Name | DNase I | ||||||
| Unit | 2000 U (1.0 ml) | ||||||
| Category | Molecular Biology Enzymes and Kits | ||||||
| Description |
DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products.
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| Application |
• Removes DNA from protein preparations |
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| Concentration | 2 U/μl | ||||||
| Note |
One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments. This product does not contain any RNase inhibitors, so it is recommended to be used in combination with RNaseOFF Ribonuclease Inhibitor (Cat. G138). This product should not be used in digestions longer than 15 minutes or at temperatures higher than 37°C, or the residual RNase activity will begin to degrade the RNA. For RNAse-Free DNAse I, see Cat. No. E091. |
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| Caution | This product is distributed for laboratory research only. Not for diagnostic use. | ||||||
| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G028 |
| How should DNase I be stored? | |
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DNase I should be stored at -20°C. To avoid repeated freeze-thaw cycles, it is recommended to aliquot the enzyme before freezing.
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| How much DNase I should I use per reaction? | |
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Typically, 1 unit of DNase I is sufficient to degrade 1 µg of DNA in 10 minutes at 37°C. Adjust enzyme concentration based on the DNA amount and reaction conditions.
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| What is the difference between this DNase I and DNase I (RNase-Free) (Cat. No. E091)? | |
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This DNase I does not contain RNase inhibitors, meaning it may have residual RNase activity that could degrade RNA. For RNA-sensitive applications, use RNase-Free DNase I (Cat. No. E091).
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| How do I prevent RNA degradation when using this DNase I? | |
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To prevent RNA degradation:
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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