DNase I (RNase-Free)
| Cat. No. | E091 | ||||||
| Name | DNase I (RNase-Free) | ||||||
| Unit | 200 U (100 μl) | ||||||
| Category | Molecular Biology Enzymes and Kits | ||||||
| Description |
DNase I is a non-specific endonuclease derived from bovine pancreas that catalyzes the cleavage of phosphodiester bonds in single/double-stranded DNA, chromatin, and RNA:DNA hybrids to generate di-and/or oligonucleotide (5’-phosphorylated and 3’-hydroxylated) end-products. This product is RNase-free and can be used in RNA applications.
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| Application |
• Removes DNA from protein preparations and RNA samples |
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| Concentration | 2 U/μl | ||||||
| Note |
One unit is defined as the amount of DNase I that catalyzes the degradation of 1 μg of DNA in 10 minutes at 37 °C into tetranucleotides or smaller fragments. Caution: Not for diagnostic use. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. E091 |
| How should DNase I be stored? | |
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DNase I should be stored at -20°C. To avoid repeated freeze-thaw cycles, it is recommended to aliquot the enzyme before freezing.
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| Can DNase I be used for RNA purification? | |
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Yes, DNase I (RNase-Free) is designed for RNA applications and can be used to remove genomic DNA contamination from RNA samples.
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| How long should I incubate my sample with DNase I? | |
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The digestion should not exceed 15 minutes to prevent RNA degradation.
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| How do I stop the DNase I reaction? | |
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The reaction can be stopped by:
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| How much DNase I should I use per reaction? | |
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Typically, 1 unit of DNase I is sufficient to degrade 1 µg of DNA in 10 minutes at 37°C. Adjust enzyme concentration based on the DNA amount and reaction conditions.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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