OneScript® Plus Reverse Transcriptase
| Cat. No. | G237 | ||||||||
| Name | OneScript® Plus Reverse Transcriptase | ||||||||
| Unit | 100 rxn | ||||||||
| Category | Reverse Transcriptase & RT-PCR | ||||||||
| Description |
OneScript® Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50°C - 55°C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript® Plus is formulated with abm’s RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT (< 1 mM), making it the best choice for ultimate RNA protection. Product Features:
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| Application |
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G237 |
| Can I use both total RNA and poly(A)+ mRNA for cDNA synthesis? | |
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Yes, both total RNA and poly(A)+ mRNA can be used, though poly(A)+ mRNA typically yields higher quantities and better purity.
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| What can I use the synthesized cDNA for? | |
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The high-quality cDNA can be used in a variety of downstream applications, including gene expression analysis, cloning, and PCR-based assays. BlasTaq™ 2X qPCR Master Mix (Cat. No. G891) is well suited to downstream qPCR applications.
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| How should I store the synthesized cDNA? | |
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Store the synthesized first-strand cDNA at -20°C for long-term use.
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| Why am I getting low cDNA yields? | |
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Low cDNA yields can be caused by poor RNA integrity, contamination, or insufficient RNA input. To improve yield:
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| How much RNA template should I use for cDNA synthesis? | |
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We recommend using 1 ng to 2 μg of RNA per reaction.
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| What volumes of cDNA should I use for downstream PCR? | |
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Typically, use 1 μl of cDNA in a 25 μl PCR reaction. You can add up to 20% of the PCR volume (e.g., 5 μl in a 25 μl PCR), depending on your target and primers.
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What if input RNA samples are expected to have high levels of genomic DNA contamination?
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If input RNA samples are expected to have high levels of genomic DNA contamination, we recommend using abm’s All-In-One 5X RT MasterMix with gDNA Removal (Cat. No. G592).
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Do I need an RNase inhibitor when using this product?
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All of abm's Reverse Transcriptases include RNaseOFF Ribonuclease Inhibitor, which protects RNA from degradation and is resistant to oxidation, even under low DTT concentrations. Therefore, there’s no need to add an external RNase inhibitor, as RNaseOFF provides optimal RNA protection during reverse transcription.
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| What discontinued abm products is Cat. No. G237 equivalent to? | |
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This product is abm’s next generation of reverse transcriptases and is functionally equivalent to Cat. No. G177, G231, and G232, with improved performance. Pair with BlasTaq™ 2X PCR MasterMix (Cat. No. G895) to achieve Two-Step RT-PCR functionality equivalent to Cat. No. G281, G282, G283, and G284 (OneScript® Two-Step RT-PCR Kits and OneScript® Plus Two-Step RT-PCR Kits). Contact our customer service team technical@abmgood.com for more information.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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