Bringing 3D biology to life.
Organ-specific, serum-free media kits for complete, validated, ready-to-use 3D culture workflows.
abm’s Spheroid & Organoid Medium Kits are a family of organ-specific, serum-free media validated and ready to use. Four distinct kits support applications spanning lung fibroblast spheroids, bronchial epithelial organoids, breast cancer tumoroids, and brain tumour spheroids, with performance-tested lots and confirmed spheroid / organoid formation data.
Spheroid & Organoid Medium Kits at a glance
No add-ons. No formulation headaches. abm’s Spheroid & Organoid Medium Kits provide chemically defined, lot-validated media from the same company that designs your cell lines.
- Product family: organ-specific, serum-free spheroid and organoid medium kits.
- Core output: reproducible 3D spheroid / organoid formation across validated cell systems.
- Research fit: pulmonary biology, COPD models, oncology screening, neural tumour models, drug response, and translational 3D biology workflows.
Why abm Spheroid & Organoid Medium Kits?
Serum-free & chemically defined
Eliminates batch-to-batch variability inherent to serum-containing formulations for reproducible results from experiment to experiment.
Xeno-free compatible
No animal-derived serum components in the formulation, helping reduce biological noise and support translational model development.
Matched cell line system
Designed and validated alongside abm’s own immortalized cell lines to help streamline model setup.
Performance-tested per lot
Every lot is validated for spheroid / organoid formation before release.
From 2D to 3D in 5 easy steps
Move from conventional 2D cell culture to organ-specific 3D spheroid and organoid workflows using a simple, ready-to-use medium kit format.
Select medium
Choose the organ-specific kit that matches your model system.
Prepare cells
Use validated companion cell lines or compatible 3D-ready cultures.
Apply medium
Begin the spheroid or organoid culture workflow with ready-to-use media.
Monitor formation
Track spheroid / organoid development and morphology over time.
Downstream use
Proceed to imaging, marker analysis, drug screening, or disease modelling.
Product Portfolio
Select from four organ-specific medium kits developed for validated spheroid and organoid formation workflows.
Lung CAF Spheroid Medium Kit
Bronchial Epithelial Organoid Medium Kit
CK7 positive bronchial epithelial organoids generated with TM601Optimized for bronchial and tracheal epithelial cells, including COPD models. Organoids express functional lung markers P63, Cytokeratin 7, and Cytokeratin 20, confirming epithelial lineage in 3D culture.
Breast Cancer Organoid Medium Kit
Brain Tumor Organoid Medium Kit
Nestin strong-positive brain organoids generated with TM603Serum-free medium supporting glioblastoma, astrocytoma, and neuroblastoma spheroid formation. U251N cells form well-defined spheroids by Day 3 and compact aggregates by Day 7, with Nestin strong-positive staining confirmed.
Validation Data – Marker Confirmation and Head-to-Head Performance
Representative validation images show lineage and marker confirmation across abm’s Spheroid & Organoid Medium Kit portfolio, along with brightfield microscopy comparisons conducted side-by-side against a leading competitor across lung, bronchial epithelial, breast cancer, and brain tumour model systems.
U251N glioblastoma astrocytoma spheroid formation
abm TM603 — Day 3, 10×
Competitor medium — Day 3, 10×
abm TM603 — Day 7, 4×
Competitor medium — Day 7, 4×U251N cells formed spheroids in both abm TM603 and a leading competitor medium by Day 3, with both developing into a similar pattern by Day 7.
BT-549 triple-negative breast cancer spheroid formation
abm TM602 — Day 7, 4×
Competitor medium — Day 7, 4×BT-549 cells cultured in TM602 for 7 days produced spheroids comparable to a leading competitor.
HCC827 bronchial / NSCLC adenocarcinoma organoid formation
abm TM601 — Day 7, 4×
Competitor medium — Day 7, 4×HCC827 cells cultured in TM601 for 7 days formed 3D aggregates under bronchial epithelial organoid workflow conditions and were compared side-by-side with a leading competitor medium.
NCI-H460 NSCLC spheroid morphology
abm TM600 — Day 5, 10×
Competitor medium — Day 5, 10×NCI-H460 cells cultured in TM600 for 5 days exhibited similar spheroid morphology to that of a leading competitor.
Ordering Information
Ready to order. All Spheroid & Organoid Medium Kits and support products are available now.
| Cat. No. | Product Name |
|---|---|
| TM600 | Lung CAF Spheroid Medium Kit |
| TM601 | Bronchial Epithelial Organoid Medium Kit |
| TM602 | Breast Cancer Organoid Medium Kit |
| TM603 | Brain Tumor Organoid Medium Kit |
| TM076 | 3DCelMatrix™ |
| G7540 | SpheroWell™ 96 Well Plate |
| G7541 | SpheroWell™ 6 Well Plate |
| G7542 | SpheroWell™ 10 cm Plate |
| G7543 | SpheroWell™ T75 Flask |
| TM078 | CryoGuard™ Freezing Media |
abm Validated Cell Companion Products
Pair each medium kit with validated cell models designed to support spheroid and organoid workflow development.
| Cat. No. | Product Name | System |
|---|---|---|
| T1013 | Immortalized Human Diploid Fibroblasts - hTERT | Lung CAF Spheroid Medium Kit |
| T0490 | Immortalized Human Pulmonary Fibroblast Cells | Lung CAF Spheroid Medium Kit |
| T9902 | NCI-H460 Cells | Lung CAF Spheroid Medium Kit |
| T0406 | Immortalized COPD Bronchial/Tracheal Epithelial Cells - SV40T | Bronchial Epithelial Organoid Medium Kit |
| T8135 | LUJ-35T Cell Line | Bronchial Epithelial Organoid Medium Kit |
| T8136 | LUJ-35T2 Cell Line | Bronchial Epithelial Organoid Medium Kit |
| T9172 | HCC827 cells | Bronchial Epithelial Organoid Medium Kit |
| T7006 | HCC38 Cells | Breast Cancer Organoid Medium Kit |
| T7012 | Hs 578T Cells | Breast Cancer Organoid Medium Kit |
| T7015 | SUM159PT Cells | Breast Cancer Organoid Medium Kit |
| T8035 | Flavopiridol-Resistant MCF-7 Cell Line (FLV1000) | Breast Cancer Organoid Medium Kit |
| T8118 | C116 Cell Line | Brain Tumor Organoid Medium Kit |
| T8242 | U251N Cells | Brain Tumor Organoid Medium Kit |
| T1008 | N/A | Brain Tumor Organoid Medium Kit / inquire for availability |
| T1157 | U251 Cells | Brain Tumor Organoid Medium Kit |
FAQ
What is the difference between a spheroid and an organoid?
Spheroids and organoids are both 3D cell culture systems, but they differ significantly in complexity and biological relevance.
Spheroids are relatively simple 3D aggregates of cells, typically one or a few cell types that self-assemble into a sphere-like structure. They are valued for their 3D microenvironment, including oxygen and nutrient gradients, cell-cell contact, and ECM interactions compared to 2D monolayers. Common examples include tumour spheroids used in oncology drug screening.
Organoids are more complex, self-organizing 3D structures derived from stem cells, either pluripotent or adult tissue-resident, that recapitulate the architecture, cell-type diversity, and functional properties of a real organ or tissue.
What are CAFs?
Cancer-associated fibroblasts are stromal cells that become activated within the tumour microenvironment. They are distinct from normal fibroblasts because they are reprogrammed by tumour-secreted signals to remodel the extracellular matrix, secrete pro-tumourigenic growth factors, suppress anti-tumour immune responses, and promote cancer cell invasion, survival, and drug resistance.
Additional Research Support
To further support 3D biology and organoid researchers, abm also provides gene tools and cell engineering services to help advance pathway discovery, model development, disease modelling, and screening workflows.
The abm Difference
More than a supplier, abm is your research partner — delivering trusted quality, rapid access, and expert support from project start to publication.
Ship-Ready Inventory
Our in-stock collection is prepared for fast fulfilment, helping you reduce downtime and move from planning to benchwork without unnecessary delays.
Scientist-to-Scientist Support
Our PhD-level team works with you directly to help identify the right model, recommend culture conditions, and support more complex study design questions.
Complete Documentation
Each order includes essential documentation such as CoA, passage history, culture guidance, and mycoplasma test reports for streamlined lab integration.
Trusted by Thousands
abm cell lines support work across oncology, pharmacology, respiratory biology, and molecular biology, helping researchers generate reliable data backed by trusted materials.