Taq 2X PCR MasterMix
| Cat. No. | G888 | ||||||
| Name | Taq 2X PCR MasterMix | ||||||
| Unit | 400 rxn (10.0 ml) | ||||||
| Category | PCR Polymerase | ||||||
| Description |
Your cost-effective choice for routine amplification! Taq 2X PCR MasterMix is a ready-to-use MasterMix containing Taq DNA Polymerase (Cat. No. G009) in a uniquely-formulated buffer with gel loading dye. Taq is a highly thermostable enzyme that catalyzes the 5’-3’ synthesis of DNA and features 5’-3’ exonuclease activity, producing 3’-dA-tailed amplicons that are ideal for TA cloning vectors. While it lacks proofreading activity due to the absence of 3'-5' activity, Taq Polymerase is known for its robust performance and reliability in generating DNA products quickly and efficiently. Its ability to perform well at high temperatures makes it an excellent choice for routine PCR applications across various research needs. Product Features:
1 MasterMix contains 1.5 mM Mg2+
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G888 |
| Does the dye in the MasterMix affect downstream applications? | |
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The loading dye does not interfere with downstream cloning experiments.
However, PCR clean-up is still advised prior to cloning due to the presence of excess primers, salts, and other buffer components that could potentially affect cloning efficiency.
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| I am observing positive results in my negative controls (no template added) when attempting to detect gene sequences in a bacterial strain. I suspect this may be due to residual bacterial DNA from the DNA polymerase. What steps should I take to address this issue? | |
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The presence of residual bacterial DNA is quite common in commercially available DNA polymerases, as most are expressed and purified using E. coli recombinant systems. In this case, we recommend switching to our Ultra-Pure BlasTaq™ 2X PCR MasterMix (Cat. No. G885). This product undergoes a rigorous multi-step purification protocol utilizing physical, chemical, and enzymatic methods to maximize the removal of contaminating genomic DNA.
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| What discontinued abm products is Cat. No. G888 equivalent to? | |
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This product is functionally equivalent to Cat. No. G013, G013-dye, G900, G900-dye (2X PCR Taq MasterMix with and without dye). Contact our customer service team technical@abmgood.com for more information.
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| How should I store this product? | |
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This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
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| Can I store my PCR reaction mix once it has been assembled? | |
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It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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