BlasTaq™ HotStart DNA Polymerase
| Cat. No. | G595 | ||||||||
| Name | BlasTaq™ HotStart DNA Polymerase | ||||||||
| Unit | 400 rxn | ||||||||
| Category | PCR Polymerase | ||||||||
| Description |
Eliminate non-specific amplification and primer-dimer formation! BlasTaq™ HotStart DNA Polymerase is a strategically-engineered, next generation Taq Polymerase that has rapid extension rates, robust performance, and contains a proprietary antibody that blocks polymerase activity at low temperatures. HotStart allows for a convenient reaction set-up at room temperature without non-specific amplification and primer dimer formation. With specialized reaction conditions, this polymerase provides increased processivity, yields, and sensitivity, while shortening reaction times by up to 70%, compared to wild-type Taq DNA polymerase. During the initial denaturation step, the antibody dissociates from the DNA polymerase and restores enzyme activity. This feature significantly reduces non-specific product formation that would otherwise compete for reagent availability offering higher specificity and improved yield of PCR products. BlasTaq™ has 5’-3’ polymerase and 5’-3’ exonuclease activities, lacks 3’-5’ exonuclease activity, and produces 3’-dA-tailed amplicons. PCR products made with BlasTaq™ can be used with TA cloning vectors. Product Features:
1 Buffer contains 1.5 mM Mg2+.
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| Storage Condition |
Store at -20°C. |
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| Material Citation | If use of this material results in a scientific publication, please cite the material in the following manner: Applied Biological Materials Inc, Cat. No. G595 |
| I am observing positive results in my negative controls (no template added) when attempting to detect gene sequences in a bacterial strain. I suspect this may be due to residual bacterial DNA from the DNA polymerase. What steps should I take to address this issue? | |
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The presence of residual bacterial DNA is quite common in commercially available DNA polymerases, as most are expressed and purified using E. coli recombinant systems. In this case, we recommend switching to our Ultra-Pure BlasTaq™ 2X PCR MasterMix (Cat. No. G885). This product undergoes a rigorous multi-step purification protocol utilizing physical, chemical, and enzymatic methods to maximize the removal of contaminating genomic DNA.
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| What discontinued abm products is Cat. No. G595 equivalent to? | |
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This product is abm’s next generation of PCR enzymes and is functionally equivalent to Cat. No. G011 and G039 (HotStart DNA Polymerase) as well as Cat. No. G937 (SensTaq HotStart DNA Polymerase), with improved performance. Contact our customer service team technical@abmgood.com for more information.
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| How does BlasTaq™ differ from regular Taq polymerase? | |
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BlasTaq™ DNA Polymerase is a next-generation, engineered Taq polymerase designed to offer faster extension rates, higher yields, and improved processivity compared to traditional wild-type Taq polymerase. It also provides enhanced sensitivity, and can reduce reaction times by up to 70% through a specialized reaction protocol.
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| How should I store this product? | |
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This product should be stored at -20°C to maintain its activity and stability. Ensure that the enzyme is kept in a frozen state until ready to use.
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| Can I use BlasTaq™ for TA cloning? | |
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Yes, BlasTaq™ DNA Polymerase produces 3’-dA-tailed amplicons, which makes it compatible with TA cloning vectors.
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| What if my PCR reaction doesn’t work well with standard conditions? | |
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If you’re working with difficult targets or crude samples, it may be necessary to increase the amount of enzyme to 1 μL for better performance. Additionally, for templates with high GC content, you can increase the initial denaturation step to 5 minutes.
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| Can I use BlasTaq™ for high-GC templates? | |
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Yes, BlasTaq™ is designed to handle a variety of templates, including those with high GC content. For high-GC templates, it is recommended to increase the initial denaturation step to 5 minutes to ensure complete denaturation of the template.
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| How do I know if my PCR reaction requires modification of the buffer or thermocycling conditions? | |
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BlasTaq™'s specialized buffer is optimized for most PCR applications, including primer annealing at 60°C. However, if your primers do not efficiently anneal or if you are working with particularly challenging templates, you may need to optimize the annealing temperature or adjust cycling conditions based on your results.
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| Can I store my PCR reaction mix once it has been assembled? | |
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It is generally not recommended to store an assembled PCR reaction for long periods, as the activity of the polymerase can decrease over time. However, you can prepare the reaction mix and store it on ice for short periods (a few hours) before running the PCR. Always prepare fresh reactions when possible for the best results.
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| What is the shelf life of BlasTaq™? | |
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The shelf life of BlasTaq™ is typically indicated on the product label or datasheet, but as a general rule, it is stable for up to 1-2 years when stored properly at -20°C. Be sure to check the expiration date and make sure the enzyme remains frozen when not in use.
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| What is the source of this enzyme? | |
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The enzyme is produced recombinantly in E. coli, which has been engineered to express the enzyme gene. While the original gene may come from another organism, all production and purification occur using E. coli under controlled conditions.
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Zahid, W., Farooqui, N., Zahid, N., Ahmed, K., Anwar, M. F., Rizwan-ul-Hasan, S., … Abidi, S. H. (2023). Association of Interferon Lambda 3 and 4 Gene SNPs and Their Expression with COVID-19 Disease Severity: A Cross-Sectional Study. Infection and Drug Resistance, 16, 6619–6628. https://doi.org/10.2147/IDR.S422095
Song, Z., Hu, K., Rao, J., Cheng, B., Xu, L., An, R., & Liang, X. (2023). Unexpected Mechanism and Inhibition Effect for Nonspecific Amplification Involving Dynamic Binding of Primers with Background DNA. Analytical Chemistry, 95(46), 16819-16829. https://doi.org/10.1021/acs.analchem.3c02274